The data which we collected during our experiment did support our hypothesis, because throughout our investigation our plant slowly withered away and lost an incredible amount of mass in the first day.
Leaf surface area- about 6 cm square 60mm square
Plant#2-Leaves shriveled, and turned slightly brown; 106.9g.
Leaf surface area-about 2cm square 20mm square
2nd Group Data(wind Only)-
Plant #1- Flower petal fall of; 66.8g.
Leaf surface area- About 5cm square 50mm square.
Plant #2- No change from yesterday; 56.5g.
Leaf surface area- About 2cm square 20mm square.
Plant #1- 89.8g
Plant #2- 100.2g
We measured transpiration using the whole plant method
Record the measurements of your plant every day throughout the entire experiment.
Plant #1 (Control)- 105.5 g
Plant #2-118.9 g
Leaf surface area- About 8cm square 80mm square
# of stomata- about 50
2nd Group Data (Wind Only)- Control- 107.2g
Plant #1- No change; 98.3g.
List of top nine experiments on transpiration in plants: ..
Figure 5.15. Testing whether photoassimilates move from mesophyll cells to se-cc complexes through (a) an entirely symplasmic route or (b) a route with an apoplasmic step. The approach is to use PCMBS as an inhibitor of membrane transport. PCMBS does not cross membranes but binds to the apoplasmic face of plasma membranes. Therefore, it blocks apoplasmic transport while symplasmic phloem loading is unaffected. PCMBS was introduced into the leaf apoplasm through the transpiration stream of excised leaves. Leaves were then exposed in a closed illuminated chamber to 14CO2. The 14C photoassimilate exported from labelled leaf blades was used to monitor phloem loading. PCMBS only reduced photoassimilate export (i.e. phloem loading) from those leaves with few plasmodesmata interconnecting se-cc complexes with surrounding cells. Thus, photoassimilate flow included a membrane transport step from the leaf apoplasm in certain plant species while others loaded via a symplasmic route. cc, companion cell; mc, mesophyll cell; msc, mesophyll sheath cell; PCMBS (para-chloromercuriben-zenesulphonic acid, also abbreviated to P); se, sieve element; vp, vascular parenchyma (Based on van Bel 1993)
Whole Plant Method Transpiration
This is important to classroom study because it reinforces the idea that many factors effect transpiration rate and this lab helps investigate which environments make the process happen faster.
[Solved] Lab #3: Plant Transpiration Worksheet - Study …
29, review water potential
Wednesday: Work on Transpiration Lab report
Friday: Read 31.1-31.4 in textbook
Review Week 4/20
Tuesday: Reach Chapter 11 and Chapter 12 and the link I sent via e-mail
2 Lab 3 Overview; 3 Plant Transpiration: Background;
By Brittany Carter
and Hart Evans “The amount of water needed daily by plants for the growth and maintenance of tissues is small in comparison to the amount that is lost through the process of transpiration.”(Lab Manual, 2001).