Aliquot and/or store the oligonucleotide probe.

The lines cannot be rinsed of whatever reagent theylast contained; thus, determine whether to continue with the synthesisbased on the reactivity of the last reagent in the lines.

In an acid buffer A and G bases are eliminated, leading to oligonucleotide damage.

The Oligonucleotide Synthesis division of the CCIB DNA Core provides investigators with custom oligonucleotides for use in a wide range of genetics applications including DNA sequencing, PCR, cDNA synthesis, hybridization, and in vitro mutagenesis. In continuous operation since 1996, our division is committed to supplying the greater Partners research community as well as non-Partners research organizations with high-quality products at low cost and with rapid turnaround time.


Syringe method for stepwise chemical synthesis of oligonucleotides.

These robust reactions allow both chemists and molecularbiologists to easily generate specific ribo and deoxy-ribo oligonucleotideswith a variety of labels, modified linkages, and non-standard bases attachedthroughout the chain.


ROPS stands for Random Oligonucleotide-Primed Synthesis

N2 - Results from a number of recent studies suggest that amino acid insertion mutations may provide an important alternative to substitution mutations for modifying protein structures and functional activities. To facilitate the use of single-amino acid insertions, we have developed a general strategy for inducing random, in-phase codon insertions across a defined segment of a cloned gene. In brief, a mixture of blocked and protected trinucleotide phosphoramidites is coupled at substoichiometric levels after every third monomer coupling on a conventional solid-state synthesizer. From the heterogeneous mixture of oligonucleotide sequences thus generated, those oligonucleotides that have acquired a single additional codon are purified by urea/PAGE. By using equimolar amounts of GCT and GGT trinucleotides in the oligonucleotide synthesis plus standard oligonucleotide-directed mutagenesis techniques, we have induced as many as 13 different single alanine and glycine insertion mutations into the gene for staphylococcal nuclease in one experiment. On replacement of the 5′-dimethoxytrityl blocking group on the trinucleotide phosphoramidite with an acid-stable blocking group, such as levulinate or fluoren-9-ylmethoxycarbonyl (Fmoc), this same strategy of substoichiometric couplings at codon boundaries should permit the synthesis of complex pools of oligonucleotides for the introduction, with constant efficiency, of every type of amino acid substitution at each codon across a gene segment.

Oligonucleotide synthesis - an overview | ScienceDirect …

AB - Results from a number of recent studies suggest that amino acid insertion mutations may provide an important alternative to substitution mutations for modifying protein structures and functional activities. To facilitate the use of single-amino acid insertions, we have developed a general strategy for inducing random, in-phase codon insertions across a defined segment of a cloned gene. In brief, a mixture of blocked and protected trinucleotide phosphoramidites is coupled at substoichiometric levels after every third monomer coupling on a conventional solid-state synthesizer. From the heterogeneous mixture of oligonucleotide sequences thus generated, those oligonucleotides that have acquired a single additional codon are purified by urea/PAGE. By using equimolar amounts of GCT and GGT trinucleotides in the oligonucleotide synthesis plus standard oligonucleotide-directed mutagenesis techniques, we have induced as many as 13 different single alanine and glycine insertion mutations into the gene for staphylococcal nuclease in one experiment. On replacement of the 5′-dimethoxytrityl blocking group on the trinucleotide phosphoramidite with an acid-stable blocking group, such as levulinate or fluoren-9-ylmethoxycarbonyl (Fmoc), this same strategy of substoichiometric couplings at codon boundaries should permit the synthesis of complex pools of oligonucleotides for the introduction, with constant efficiency, of every type of amino acid substitution at each codon across a gene segment.

DNA-templated oligonucleotide synthesis using ..

We offer a convenient and free delivery service to our remote drop-box locations (via Courier or core personnel). Oligonucleotides can also be picked up directly at our facility.