(PHA) Biosynthesis from Whey Lactose

Polyhydroxyalkanoates (PHAs) are polyesters synthesized by numerous bacteria and storaged by the form of cytoplasmatic inclusions as energy reserve material and reducing power under certain unbalanced growth conditions (Anderson and Dawes, 1990; Lee and Choi, 1999). The composition of PHAs depends mainly on the PHA synthases, the , the cultivation conditions and the metabolic routes involved (Hoffmann ., 2000). Economic evaluation of the process for the production of P(3HB) suggested that the major contributor of the overall 3(PHB) production cost was carbon substrate cost (up to 50%) (Choi and Lee, 1997).

(PHA) Biosynthesis from Whey Lactose.

Table Abbreviations: CDM: Cell dry mass; GBL: γ-Butyrolactone; Hfx: Haloferax; mcl: medium chain length; PHA: Polyhydroxyalkanoate; PHB: Poly (3-hydroxybutyrate); scl: short chain length

Polyhydroxyalkanoate (PHA) biosynthesis from whey lactose ..

Archaeal Production of Polyhydroxyalkanoate (PHA) Co …

µmax: Maximum Specific Growth Rate; 16S rRNA: 16 Svedberg rRibosomal RNA Sequencing; 3HB: 3-HydroxyButyrate; 3HD: 3-HydroxyDecanoate; 3HDD: 3HydroxyDocecanoate; 3HHx: 3-HydroxyHexanoate; 3HN: 3-HydroxyNonanoate; 3HO: 3-HydroxyOctanoate; 3HV: 3HydroxyValerate; 3UD: 3-HydroxyUnDecanoate; 4HB: 4-HydroxyButyrate; AC: Acetyl-CoA synthetase; BOD: Biochemical Oxygen Demand; b-PHA: Blocky Structured PolyhydroxyAlkanoate; CBF: Cyclic Batch Fermentation; CDM: Cell Dry Mass; CFBF: Cyclic Fed-Batch Fermentation; COD: Chemical Oxygen Demand; CT: CoA Transferase; DSC: Differential Scanning Calorimetry; ECS: Extruded Corn Starch; EPS: Extracellular Polysaccharides; ERB: Extruded Rice Bran; FT-IR: Fourier Transform Infrared Spectroscopy; GBL: γ-ButyroLactone; GC-MS: Gas Chromatography coupled to Mass Spectroscopy; Hm: Melting Enthalpyk; Da: 103 Dalton; LPS: Lipopolysaccharides; MCL: Medium Chain Length; MW: Weight Average Molecular Mass; NADH: Nicotinamide Adenine Dinucleotide; NADPH: Nicotinamide Adenine Dinucleotide Phosphate; NMR: Nuclear Magnetic Resonance; PCR: Polymerase Chain Reaction; PHA: PolyHydroxyAlkanoate; PHB: Poly (3-HydroxyButyrate); PHBHV: Poly (3-HydroxyButyrate-co-3-HydroxyValerate); PHV: Poly (3-HydroxyValerate); Pi: Polydispersity; qp: Specific Production rate; scl: Short Chain Length; SDS: Sodium Dodecyl Sulfate; SEM: Scanning Electron Microscopy; SSCAs: Star-Shaped Cell Aggregates; STEM: Scanning Transmission Electron Microscopy; TCA: Citric Acid Cycle; TDS: Total Dissolved Solids; TEM: Transmission Electron Microscopy; Td: Onset of polymer Decomposition Temperature; Tg: Glass Transition Temperature; Tm: Melting Temperature; Xc: Degree of Crystallinity