Plant Pigment Chromatography ..


Because different pigments absorb light of different wavelengths, by
comparing the occurrence of different pigments we can deduce the
plants' habitat;

l We can do further experiment to determine the absorption spectrum of
each pigment.

06/12/2015 · Photosynthesis Lab: Plant Pigment Chromatography and Light Reactions ..

Green plant have six closely related photosynthesis pigments(in order of increasing polarity): Carotene (orange pigment),XanthophyII (yellow pigment), Phaeophytin
a
(grey-brown pigment),Phaeophytin
b
(yellow-brown pigment), ChlorophyII
a
(blue-green pigment) and ChorophyII
b
(yellow-green pigment)
The retention factor (R
f)
may be defined as the ratio of the distance traveled by the substance to the distance traveled by the solvent
3.0 METHODOLOGY
3.1 MATERIAL AND APPARATUS
3.2 ARRANGEMENT OF APPARATUS
CHEMISTRY
PBS

How to identify the presence of difference pigments in chlorophyll's
What are the factors affecting these R
f
values.
PIGMENT SEPARATION
USING PAPER CHROMATOGRAPHY

80% of Acetone
"Cekur Manis" or Red Spinach Leaves
Mortar and Pestle
Muslin (cheese cloth)
Boiling Tube/Large Test Tube With Stopper (24x150mm)
Solvent ( 1 Part 80% Acetone and 9 Part Petroleum Ether)
Ruler
Whatman no.1 Chromatography Paper Strip (20 X 130mm)
Siccors
Pin
Dark Paper/Black Sugar Paper
Retort Sand/ Test Tube Rack
Diagram 1: Assembling of chromatography apparatus
3.3 PROCEDURE
1.


Plant Pigment Chromotography Paper chromatography is a ..

BISC110/S10: Series 3 Experiment 8 Photosynthetic Pigments

Last but not least to record all the data in a table.
4.0 DATA INTERPRETATION AND DICUSSION
Band Colour
Plant Pigment
Distance moved by the pigment (mm)
Distance moved by the solvent (mm)
R
f
value
Green
ChlorophyII a
31
109
31/109=0.28
Blue Green
ChlorophyII b
80
109
80/109=0.73
Yellow
XanthophyII
86
109
86/109=0.79
Grey
Phaeophytin
92
109
92/109=0.84
Yellow
Orange
Carotene
102
109
102/109=0.94
4.1 RESULT/TABULATION OF DATA
4.0 DISCUSSION
From the experiment,the pigments that exist in the plant based on their colors were chlorophyll a which show green color, chlorophyll b which was blue green,xanthophyII which was grey and carotene which was orange in color.
the factors that affecting the movement of pigment during chromatography were porosity of the chromatography paper,solubility of the solvent as well as the molecular size of the solute.
the pigment which moved the furthest was carotene by 102 nm in distance, this indicates that the soluble pigment carotene dissolved into the acetone easiest and thus moved furthest.
it is very soluble in the solvent being used and because it forms no hydrogen bond with cellulose.
beside the large value of R
f
value also indicates that carotene was smallest in size.
The molecular size of the pigment is inversely proportional to the R
f
value.
Conclusion
Precautions
To obtain a clear and accurate chromatogram the students were advised to use gloves.