The yeast seed culture prepared as described above was inoculated at 5% inoculum size into four 3 L capacity fermentors (Bioflo 110, New Brunswick Scientific, Edison, NJ, USA) each containing 2 L of the optimum fermentation medium with 2.5% (w/v) yeast extract as nitrogen source at pH 5.5 and cultured under the optimum fermentation conditions obtained from response surface methodology. After fermentation, yeast cells were removed by centrifugation, and the supernatant was rotary decolorized with activated carbon at 80°C. Then the colorless fermentation broth thus obtained was clarified by removal of activated carbon via filtration, and was applied to ion-exchange resin columns for removal of metal ions, which was verified by a conductivity less than 20 s/cm. The solution was adjusted to contain 70% (w/v) of L-arabinose by concentrating, and cooled gradually from 70°C to 4°C to allow L-arabinose crystallized. White powdery crystals precipitated from solution, then were centrifuged and washed with 95% ethanol, and dried. The purity of L-arabinose was estimated by HPLC.
A new approach to the synthesis of optically active pseudo-sugar and pseudo-nucleoside: Synthesis of pseudo- D-arabinofuranose, (dextro)-cyclaradine, and (dextro)-1-pseudo- D-arabinofuranosyluracil from D-arabinose.
NEW SYNTHESIS OF L-FMAU FROM L-ARABINOSE - …
L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus making high production cost. Therefore, there is a biotechnological and commercial interest in the development of new cost-effective and high-performance methods for obtaining high purity grade L-arabinose.