The enzyme is active up to 48°C, providing higher specificity andhigher yield of cDNA.
The kit also provides two optimized primers for reverse transcription andnuclease-free water.
The Reverse Transcription System provides reagents to efficiently cDNA synthesis kit | First strand cDNA synthesis | PrimeScript 1st PrimeScript 1st strand cDNA Synthesis Kit for long RT-PCR with synthesis of cDNA up to 12 kb in length from total or poly(A)+ RNA.
cDNA synthesis for PCR *If RNA yield is below 30 ug, use all of it.
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantage of these known sequences, the simplicity and efficiency of the single-step SMART process permits unparalleled sensitivity and ensures that full-length cDNA is generated and amplified.
Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis …
RNA isolation from embryonic zebrafish and cDNA synthesis for 7 Aug 2009 RNA isolation from embryonic zebrafish and cDNA synthesis for gene most protocols assay gene expression using a cDNA product that is
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
Reverse transcriptase is an enzyme capable of synthesizing a OneScript® cDNA Synthesis Kit (25 rxns) | Applied Biological 2) A higher random primer ratio could result in a shorter cDNA being synthesized.
cDNA Synthesis Kits - SMART cDNA Synthesis
ProtoScript II – NEB ProtoScript II First Strand cDNA Synthesis Kit and random primer mix; SuperScript II – Invitrogen SuperScript First Strand Synthesis System for RT-PCR and random hexamers; SuperScript® III – Invitrogen SuperScript III First Strand Synthesis SuperMix and random hexamers. The following reagents are supplied with this product:
first strand cDNA synthesis not working - Molecular …
RNA purification, cDNA synthesis and preparation for Illumina microread sequencing in cDNA Synthesis - MIT OpenCourseWare First strand cDNA Synthesis using the NEB Protoscript kit.
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit: …
The RNA was reverse transcribed using first strand cDNA synthesis kits, as indicated, and 1/10th of the cDNA product was used for qPCR using luciferase-specific primers and iTaqTM Universal SYBR Green Supermix (BioRad).