The addition of human recombinant interleukin-1β (IL-1β) to cultures of lapine articular chondrocytes provoked the synthesis of large amounts of NO and reduced the production of type-II collagen. N(G)-Monomethyl-L-arginine (L-NMA), an inhibitor of NO synthase, strongly suppressed the production of NO and partially relieved the inhibition of collagen synthesis in response to IL-1β. The NO donor S-nitrosoacetylpenicillamine (SNAP), on the other hand, inhibited collagen production. IL-1 lowered the abundance of Col2A1 mRNA in an NO-independent manner. Collectively, these data indicate that IL-1 suppresses collagen synthesis at two levels: a pretranslational level which is NO-independent, and a translational or post-translational level which is NO-mediated. These effects are presumably specific as L-NMA and SNAP had no effect on total protein synthesis or on the distribution of newly synthesized proteins between the cellular and extracellular compartments. Prolyl hydroxylase is an important enzyme in the post-translational processing of collagen, and its regulation and cofactor requirements suggest possible sensitivity to NO. Extracts of cells treated with IL-1 or SNAP had lower prolyl hydroxylase activity, and L-NMA was partially able to reverse the effects of IL-1. These data suggest that prolyl hydroxylase might indeed be a target for NO. Because under-hydroxylated collagen monomers fail to anneal into stable triple helices, they are degraded intracellularly. Inhibition of prolyl hydroxylase by NO might thus account for the suppressive effect of this radical on collagen synthesis.
Remember, we learned our collagen production is decreasing by 1% a year? The boost in collagen that Trylagen PCB stimulates is vital to help us combat that natural decrease…
Proline Precursors to Sustain Mammalian Collagen Synthesis
Presentation Summary : Increases synthesis of inflammatory serum ... Composed primarily of collagen to restore the strength of the tissue but not its ... Chain of Survival and EMSC
Glossary | Linus Pauling Institute | Oregon State University
Presentation Summary : The Phoenix laser system stimulates fibroblast development and accelerates collagen synthesis in damaged tissue. ... Treatment Training Last modified by:
Synthesis and Structure Inside fibroblasts
Presentation Summary : Stimulates both endochondral and intramembranous bone formation and collagen type 2 synthesis. ... Fracture healing and wound healing Last modified by:
pre-pro-collagen α chain formation
CCK travels through the bloodstream and binds to receptor cells in the acini of the pancreas. CCK stimulates these cells to produce and secrete pancreatic juice that has a high concentration of digestive enzymes. The high levels of enzymes in pancreatic juice help to digest large protein and lipid molecules that are more difficult to break down. Pancreatic health problems. If the ducts leading from the pancreas are blocked in some way – such as when a gallstone blocks the ampulla of Vater - pancreatic fluids can build up in the pancreas and may then become activated so that they digest the pancreas itself.
Firming Collagen Serum - Skin Actives Scientific
Presentation Summary : Medical and Psychosocial Aspects of Rehabilitation Counseling ... of collagen collagen synthesis ... RCS 6080 Medical and Psychosocial Aspects of ...