Instead of the random hexamers you can use 4 pmole gene-specific primers or 1 µg Oligo(dT)12-18 (each given as total amount for this reaction mix). I prefer the random hexamers, because one synthesis can be used for whatever you want to detect and is not enriching the 3' ends of the RNA. Furthermore, it just works well.
DDT is a remarkable molecule, since it kills a wide variety of insect pests, such as houseflies, body lice, mosquitoes, Colorado beetles, and gypsy moths. This activity is heightened by the fact that it has little or no toxicity to mammals or other animals, and is a very stable molecule that can be manufactured by a simple and cheap process. This process is almost the ideal organic synthesis - heat the reactants together in a single step, pour the reaction mixture into water and filter off the DDT, which can be used without further purification!
This process is almost the ideal organic synthesis ..
An oxygen switch in catalysis of the cobalamin derivative (B12)-TiO2 hybrid catalyst for the dechlorination of trichlorinated organic compounds have been developed. The covalently bounded B12 on the TiO2 surface transformed the trichlorinated organic compounds into an ester and amide by UV light irradiation under mild conditions (in air at room temperature), while dichlorostilbenes (E and Z forms) were formed in nitrogen from benzotrichloride. A benzoyl chloride was formed as an intermediate of the ester and amide, which was detected by GC-MS. The substrate scope of the synthetic strategy is demonstrated with a range of various trichlorinated organic compounds. A photo-duet reaction utilizing the hole and conduction band electron of TiO2 in B12-TiO2 for the amide formation was also developed.