seedlings and LIMYB-mediated inhibition of protein synthesis

Application Notes
Cycloheximide is a glutarimide antibiotic derived from Streptomyces griseus. It is active against most yeast and fungi and is often used in bacteriological media for isolation and counting of unknown bacteria. It has been reported that cyclohexmide inhibits eukaryotic protein synthesis.2 Cycloheximide has also been used as a plant growth regulator by inducing the production of ethylene in plants.3, 4

The function of the Golgi Apparatus and Endoplasmic reticulum in protein synthesis.

In tissues of green plants, differential effects have beenthought to be due to a preferential inhibition by chloramphenicol ofprotein synthesis mediated by 70S ribosomes as found in chloroplasts,while cycloheximide inhibits syntheses mediated by 80S cytoplasmicribosomes1-3 to a greater extent.


Inhibition of Protein Synthesis and ..

Inhibition of Protein Synthesis by Cyclo-heximide (Actidione) in Chlorella

SAHA induces Tie2 protein degradationvia the ubiquitin/proteasome pathway. (A) Cells were cultured inmedia containing various concentrations of SAHA for 24 h and thentreated with 10 μg/ml of cycloheximide (CHX) to block proteinsynthesis. Cellular proteins were harvested at different timesafter cycloheximide addition, and the Tie2 protein level wasdetected by western blotting. (B) The Tie2 protein level in thecells collected at 4, 9 and 13 h was compared to the Tie2 level attime zero (defined as 100%). (C) Cells were co-treated withdifferent concentrations of SAHA and MG-132 (proteasome inhibitor),chloroquine (autophagy inhibitor) or leupeptin (proteaseinhibitor). The Tie2 protein level was investigated by westernblotting.


Beating Colon Cancer with Artemisinin

Itinhibits the growth of fungi1,2, algae3,protozoa4 and higher plants5, but has no effect onbacterial growth1.

HUMAN EXPOSURE ASSESSMENT - IPCS INCHEM

Cells were cultured in the media containing variousconcentrations of SAHA for 24 h and then treated with 10 μg/ml ofcycloheximide to block protein synthesis. Cellular proteins wereharvested at different times after cycloheximide addition, and theTie2 protein level was detected by western blotting. Tie2 proteinlevel in the cells collected at time zero was defined as 100%.

Full text of "NEW" - Internet Archive

Withintact cells, it was not possible to resolve the controversy overwhether the primary effect of cycloheximide was on the synthesis ofdeoxyribonucleic acid or on the synthesis of protein.

01/02/2016 · Results

SAHA induces Tie2 protein degradationvia the ubiquitin/proteasome pathway. (A) Cells were cultured inmedia containing various concentrations of SAHA for 24 h and thentreated with 10 μg/ml of cycloheximide (CHX) to block proteinsynthesis. Cellular proteins were harvested at different timesafter cycloheximide addition, and the Tie2 protein level wasdetected by western blotting. (B) The Tie2 protein level in thecells collected at 4, 9 and 13 h was compared to the Tie2 level attime zero (defined as 100%). (C) Cells were co-treated withdifferent concentrations of SAHA and MG-132 (proteasome inhibitor),chloroquine (autophagy inhibitor) or leupeptin (proteaseinhibitor). The Tie2 protein level was investigated by westernblotting.