Sucrose has been considered as an important factor regulating growth and metabolism of plants. For the cultivation and maintenance of plants , 3% sucrose has been recommended optimum. In view of this, we evaluated the impact of low and high sucrose content on the leaf morphology and steviol glycoside biosynthesis pathway during normal photoperiod in .
Expression analysis of genes of steviol glycoside biosynthesis pathway in response to sucrose variability: In order to have an account of sucrose variability on steviol glycoside biosynthesis pathway, the transcript expression of seven genes of the pathway was analyzed; CDPS (copalyl diphosphate synthase), KS (kaurene synthase), KO (kaurene oxidase), KAH (kaurenoic acid-13-hydroxylase) and three UGTs (UDP-glycosyltransferases) UGT85C2, UGT74G1 and UGT76G1.
Biosynthesis and Metabolism of Sucrose - …
Castleden CK, Aoki N, Gillespie VJ, et al. (2004) Evolution and function of the sucrose‐phosphate synthase gene families in wheat and other grasses. Plant Physiology 135: 1753–1764.
Biosynthesis of Sucrose with Sugar Cane Leaf Preparations
From times till date, studies have well documented the multifarious important functions of sucrose during plant development from their germination to maturity. Sucrose has been reported to enter into various metabolic pathways and initiate release of energy wherever required. In addition, sucrose also acts as gene regulator (). An interesting fact regarding sucrose responsiveness is that a specific concentration of sucrose is a necessity for plant growth and survival and at the same time mere increase or decrease from the normal concentration produces adverse results. It has been suggested that plants respond to changing sucrose content by undergoing morphological and anatomical variations as well as by regulating the expression of various genes via a variety of pathways (; ; ).
Chapter 19 : Carbohydrate Biosynthesis
Thus, sucrose has been considered an important factor assisting plant growth, survival and maintenance. Keeping in view the importance of steviol glycoside biosynthesis pathway and regulatory aspects associated with exposure of sucrose, present research aims to study the influence of variable sucrose concentration on the transcript expression pattern and metabolite production of steviol glycoside biosynthesis pathway in .
At this point the focus shifts entirely to plants
Expression analysis of steviol glycoside biosynthesis pathway gene transcripts: For evaluating the influence of variable sucrose content on steviol glycoside biosynthesis pathway, transcript analysis of genes CDPS, KS, KO, KAH, UGT85C2, UGT74G1 and UGT76G1 was carried out. Total RNA was isolated from young leaf tissues of 40 days old plants. Total RNA was isolated using Qiagen RNeasy Plant Minikit as per manufacturers instructions. RNA was quantified using Nanodrop ND-1000 (Nanodrop Technologies, USA). cDNA was synthesized using 50 ng of RNA in the presence of 100 U Superscript III Reverse Transcriptase (Invitrogen), 1 μL of 10 mM dNTPs mix and 250 ng oligo-(dT)12-18, respectively. Two μL of the synthesized cDNA was utilized as template to study the expression profile of steviol glycoside biosynthesis pathway genes via semi-quantitative reverse transcription PCR.
A novel levansucrase was characterized from B
Subbaiah CC, Palaniappan A, Duncan K, et al. (2006) Mitochondrial localization and putative signalling function of sucrose synthase in maize. Journal of Biological Chemistry 281: 15625–15635.