SensiFAST™ cDNA Synthesis Kit - Bioline

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Sinapse Biotecnologia - Sensifast cDNA Synthesis Kit - Bioline

The kit combines a reverse transcriptase and a proprietary hot-start DNA polymerase, for high PCR specificity and sensitivity of the one-step RT-PCR.

The kit generates real-time ready first strand cDNA from viral RNA or total RNA and owes much of its high performance to the TransAmp™ buffer. This novel buffer includes a highly optimized blend of random and anchored oligo(dT) primers, to ensure RNA is accurately represented without bias in the generated cDNA. Furthermore, it is designed to overcome challenging secondary structures in a 42oC reaction, which is the optimal temperature for the new enzyme. Similar to SensiFAST™ real-time kits, the cDNA synthesis kit is both sensitive and fast, producing a valid representation of low copy RNA transcripts in a rapid 30 minute protocol.