Click Chemistry -- Publications

With the appropriate conditions in hand, we proceeded to construct a combinatorial library of trimeric PTAs following an invariant linker. The synthesis was conducted on TentaGel beads, which is the preferred resin for subsequent screening (). 2-Bromoacetic acid, (S)-2-bromopropanoic acid-d4 and (R)-2-bromopropanoic acid) were employed as sub-monomers along with 16 amines (see ). The theoretical diversity of the library was 110,592 compounds. The amines were chosen carefully such that any combination of amine and acid sub-monomers, could be identified uniquely by tandem mass spectrometry upon gas phase cleavage of the amide bond. Methionine was added to the beads first using standard peptide coupling conditions (HBTU, Fmoc-Met-OH, DIEA) to facilitate selective release of the compounds from the beads after the screen by treatment with CNBr. Following the methionine, several invariant peptoid residues were added. The two Nlys residues facilitate display of the molecule in aqueous solution while the alkyne and furan side chains facilitate subsequent labeling of the compound () and immobilization on a microarray () if desired. Moreover, the additional mass serves to move the molecular ion and important fragment peaks out of the range of the matrix peaks, which facilitates compound characterization.

A Methionine-Based Linker Strategy for Solid-Phase Synthesis of Lysine-Containing Cyclic Peptides.

Of course, the same arguments would apply to molecules containing other N-substituents besides methyl. Thus it would be of great interest to construct combinatorial libraries of diverse N-alkylated peptides and investigate them as sources of bioactive molecules, but this has never been done. N-methylated, N-protected amino acid building blocks are available (), providing the building blocks required for the synthesis of OBOC libraries of N-methylated peptides by standard peptide bond couplings. But to apply a standard peptide synthesis approach to libraries with different N-substituents would entail the maintenance of a huge number of different N-substituted, Fmoc-protected amino acid building blocks. To address this important goal, we therefore considered a different solution: to create libraries of PTAs via sub-monomer synthesis in which chiral 2-bromo carboxylic acids are employed in place of 2-bromoacetic acid (; R1 ≠ H).

Biotage - Biotage® Initiator+

In peptide synthesis, microwave irradiation has been used to complete long peptide sequences with high degrees of yield and low degrees of racemization. Microwave irradiation during the coupling of amino acids to a growing polypeptide chain is not only catalyzed through the increase in temperature, but also due to the alternating electromagnetic radiation[citation needed] to which the polar backbone of the polypeptide continuously aligns to. Due to the this phenomenon, the microwave energy can prevent aggregation and thus increases yields of the final peptide product. There is however no clear evidence that microwave is better than simple heating and some peptide laboratories regard microwave just as a convenient method for rapid heating of the peptidyl resin. Heating to above 50-55 degrees celcius also prevents aggregation and accelerates the coupling.

Chymotrypsin - an overview | ScienceDirect Topics

Cyclic peptides and depsipeptides are a class of privileged molecular structures. In comparison to linear peptides, cyclic peptides are more stable against proteolytic degradation due to their lack of free N- or C-terminus and reduced conformational freedom. The entropic advantages associated with the increased rigidity also make cyclic peptides tighter-binding and potentially more specific ligands of macromolecular receptors. In addition, cyclic peptides are more bioavailable (e.g., greater membrane permeability) than linear peptides due to the absence of N- and C-terminal charges and their ability to form intramolecular hydrogen bonds as they traverse the lipid bilayer. Not coincidentally, cyclic peptides and depsipeptides are widely distributed in nature and possess a broad range of biological activities.- Several cyclic peptides such as cyclosporin A (an immunosuppressant), caspofungin (an antifungal agent), and daptomycin (an antibiotic) are clinically used therapeutic agents. Given their potential as drugs, drug leads, and molecular tools in biomedical research, there has been much interest in the generation of cyclic peptide natural product analogues, either chemically- or enzymatically,- to further improve their native activities or to impart new activities. In the previous studies, cyclic peptide analogues were generally prepared through sequential or parallel synthesis, which practically limited the size of libraries to 102−103 variants. In principle, much larger cyclic peptide libraries (up to 107 variants) should be readily accessible via combinatorial synthesis by the split-and-pool method., However, combinatorial synthesis necessitates post-screening sequence determination of “hit” peptides; until very recently, sequence determination of cyclic peptides had been a very challenging problem.

Facile Method To Sequence Cyclic ..

The structure of a peptide is determined by its sequence and the chemical environment in which it is placed. Linear peptides are relatively unrestrained and are able to adopt a range of conformations that are not always ideal for some applications. Cyclizing the peptide can restrain these conformations and increase the utility of the peptide in certain applications. One such method is to form a disulfide bridge between two Cysteine residues. Mimotopes routinely carries out this reaction and has considerable experience in the formation of these disulfide bridges.

Cyclic structures occur naturally in proteins and are also often the designed structure in phage random peptide library screening. We can cyclize your peptide if it contains two Cysteine residues. Other unusual thiol containing residues can also be used to cyclize your peptide. Peptides with two or more Cysteine residues can polymerize or cyclize in unwanted ways and it is likely the final product will contain a mixture of various polymers and cyclic peptide conformations. Typically the highest purity we offer for peptides ordered with a disulfide bond is >80%.